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DNA polymerases. --- Polymerase chain reaction. --- Investigative techniques --- Genetic techniques --- Polymerase chain reaction --- Molecular biology --- methods
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Nucleic acids --- Molecular Diagnostic Techniques --- Nucleic Acids --- Genetic Techniques --- Clinical Laboratory Techniques
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Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Cloning. --- Birds --- Mammals --- Genetics. --- Aves --- Avian fauna --- Avifauna --- Wild birds --- Amniotes --- Vertebrates --- Ornithology --- Genetic engineering --- Reproduction, Asexual --- Animal genetics. --- Cytology. --- Developmental biology. --- Medicine. --- Animal Genetics and Genomics. --- Cell Biology. --- Developmental Biology. --- Molecular Medicine. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Development (Biology) --- Biology --- Growth --- Ontogeny --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Genetics --- Health Workforce --- Cell biology. --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Genetic Techniques. --- Genetic Techniques --- Medicine --- Genetics and Genomics. --- Developmental Biology and Stem Cells. --- Biomedical Research. --- Research. --- Biological research --- Biomedical research --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology)
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Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.
Life Sciences. --- Microbiology. --- Microbial Genetics and Genomics. --- Microbial Ecology. --- Life sciences. --- Microbial ecology. --- Microbial genetics. --- Sciences de la vie --- Ecologie microbienne --- Microbiologie --- Génétique microbienne --- Bacteria -- Classification. --- Organisms --- DNA --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Nucleic Acids --- Genetic Techniques --- Bacteria --- DNA, Bacterial --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- 579.23 --- Morphology and cytology of microorganisms. Prokaryotic cells --- 579.23 Morphology and cytology of microorganisms. Prokaryotic cells --- Environmental microbiology --- Microorganisms --- Ecology --- Microbiology --- Bacterial systematics --- Bacterial taxonomy --- Bacteriology --- Prokaryotes. --- Prokaryotes --- Ultrastructure. --- Monera --- Procaryotae --- Procaryotes --- Prokaryonta --- Prokaryotae --- Prokaryotic protists --- Microbial genomics. --- Ultrastructure (Biology) --- Genomics --- Microbial genetics --- Genetics --- Microbial biology --- Microbial ecology
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It is now well recognized that individualized cancer treatment planning, based on tumor characteristics specific to an individual's cancer, makes it easier to select those most likely to benefit from toxic cancer therapies and to avoid treating those least likely to benefit. In Biomarkers in Breast Cancer: Molecular Diagnostics for Predicting and Monitoring Therapeutic Effect, expert laboratory and clinical researchers from around the world review how to design and evaluate studies of tumor markers, as well as examine their use in breast cancer patients. The authors cover both the major advances in sophisticated molecular methods and the state-of-the-art in conventional prognostic and predictive indicators. Among the topics discussed are the relevance of rigorous study design and guidelines to the validation of new biomarkers, gene expression profiling by tissue microarrays, adjuvant systemic therapy, and the use of estrogen, progesterone, and epidermal growth factor receptors as both prognostic and predictive indicators. Highlights include the evaluation of HER2 and EGFR family members, of p53, and of UPA/PAI-1; the detection of rare cells in blood and marrow; and the detection and analysis of soluble, circulating markers. Authoritative and cutting-edge, Biomarkers in Breast Cancer: Molecular Diagnostics for Predicting and Monitoring Therapeutic Effect offers laboratory investigators developing new tumor markers, clinical investigators testing them, and clinicians using them an up-to-date understanding of both the prognostic/predictive indicators and the novel molecular-targeted therapies suitable for individualizing breast cancer therapy.
Breast Neoplasms --- Molecular Diagnostic Techniques. --- Tumor Markers, Biological. --- Breast --- Tumor markers. --- Sein --- Marqueurs tumoraux --- diagnosis. --- Cancer --- Molecular diagnosis. --- Prognosis. --- Pronostic --- Breast -- Cancer -- Molecular diagnosis. --- Breast -- Cancer -- Prognosis. --- Tumor markers --- Breast Diseases --- Clinical Laboratory Techniques --- Neoplasms by Site --- Genetic Techniques --- Investigative Techniques --- Skin Diseases --- Neoplasms --- Diseases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Skin and Connective Tissue Diseases --- Molecular Diagnostic Techniques --- Medicine --- Health & Biological Sciences --- Oncology --- Molecular diagnosis --- Prognosis --- Biological markers (Oncology) --- Cancer markers --- Markers, Tumor --- Tumor associated markers --- Medicine. --- Cancer research. --- Biomedicine. --- Cancer Research. --- Cancer research --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Biochemical markers --- Tumors
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In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.
In situ hybridization --- In Situ Hybridization --- Laboratory manuals --- Electronic books. -- local. --- In situ hybridization -- Laboratory manuals. --- Nucleic acid hybridization. --- Laboratory Manuals --- Staining and Labeling --- Publication Formats --- Nucleic Acid Hybridization --- Publication Characteristics --- Histocytological Preparation Techniques --- Genetic Techniques --- Investigative Techniques --- Cytological Techniques --- Histological Techniques --- Clinical Laboratory Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetics --- Biology --- Health & Biological Sciences --- Cytogenetics --- Cytology --- Hybridization --- Nucleic acid hybridization --- Technique --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- In situ hybridization - Laboratory manuals --- In Situ Hybridization - laboratory manuals
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*Analysis of Genes and Genomes* is a clear introduction to the theoretical and practical basis of genetic engineering, gene cloning and molecular biology. All aspects of genetic engineering in the post-genomic era are covered, beginning with the basics of DNA structure and DNA metabolism. Using an example-driven approach, the fundamentals of creating mutations in DNA, cloning in bacteria, yeast, plants and animals are all clearly presented. Newer technologies such as DNA macro and macroarrays, proteomics and bioinformatics are introduced in later chapters helping students to analyse and understand the vast amounts of data that are now available through genome sequence and function projects. Aimed at students with a basic knowledge of the molecular side of biology, this will be invaluable to those looking to better understand the complexities and capabilities of these important new technologies. - A modern post-genome era introduction to key techniques used in genetic engineering. - An example driven past-to-present approach to allow the experiments of today to be placed in an historical context - Beautifully illustrated in full colour throughout. - Associated website including updates, additional content and illustrations
577.1 --- 577.1 Chemical bases of life. Biochemistry and bio-organic chemistry generally --- Chemical bases of life. Biochemistry and bio-organic chemistry generally --- gisten --- yeast [fungi] --- Genome --- Genetic engineering --- Molecular genetics --- Genetics --- Molecular biology --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Research&delete& --- Methodology --- moleculaire biologie --- genetica --- Nuclear physics --- polymeren --- Macromolecules --- DNA (deoxyribonucleic acid) --- Genetic Techniques --- DNA --- Research --- analysis --- bio-informatica --- genomics --- stamcellen --- klonen --- mutageniteit --- Escherichia coli --- proteomics --- oligonucleotides --- enzymen --- electrophoresis --- insecten --- Escherichia --- PCR (polymerase chain reaction) --- Molecular genetics - Research - Methodology --- Genetic engineering - Research - Methodology --- DNA - analysis --- Acqui 2006
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Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
Cell Nucleus --- Cloning, Organism --- Cell Differentiation --- Gene Transfer Techniques. --- Cell nuclei --- Gene therapy. --- Transgenic animals. --- Thérapie génique --- Animaux transgéniques --- transplantation. --- methods. --- genetics. --- physiology. --- Transplantation. --- Gene therapy --- Transgenic animals --- Gene Transfer Techniques --- Transplantation --- methods --- genetics --- Cell nuclei -- Transplantation. --- Investigative Techniques --- Surgical Procedures, Operative --- Genetic Techniques --- Publication Formats --- Cell Physiological Processes --- Biology --- Biological Science Disciplines --- Reproductive Techniques --- Intracellular Space --- Organelles --- Cell Physiological Phenomena --- Publication Characteristics --- Cytoplasmic Structures --- Natural Science Disciplines --- Cellular Structures --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Cells --- Cytoplasm --- Phenomena and Processes --- Anatomy --- Laboratory Manuals --- Methods --- Physiology --- Genetics --- Medicine --- Health & Biological Sciences --- Cytology --- Pathology --- Therapy, Gene --- Nuclear transfer --- Nuclear transplantation --- Transplantation of cell nuclei --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Animals --- Transgenic organisms --- Animal genetic engineering --- Genetic engineering --- Therapeutics --- Reproduction --- Cytology. --- Cell nuclei - Transplantation --- Cloning, Organism - methods - laboratory manuals --- Cell Differentiation - genetics - laboratory manuals --- Gene Transfer Techniques - laboratory Manuals
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Genomics --- Gene mapping --- Physiology, Comparative --- Computational biology --- Génomique --- Cartes chromosomiques --- Physiologie comparée --- Bio-informatique --- Congresses. --- Statistical methods --- Congrès --- Méthodes statistiques --- Congresses --- Computational Biology --- Chromosome Mapping --- Biology --- Genetics --- Genetic Techniques --- Physiology --- Investigative Techniques --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Computer Science --- Engineering & Applied Sciences --- Health & Biological Sciences --- Bioinformatics --- Chromosome mapping --- Genetic mapping --- Genome mapping --- Linkage mapping (Genetics) --- Mapping, Gene --- Life sciences. --- Data structures (Computer science). --- Algorithms. --- Computer science --- Database management. --- Biochemistry. --- Bioinformatics. --- Life Sciences. --- Biochemistry, general. --- Algorithm Analysis and Problem Complexity. --- Discrete Mathematics in Computer Science. --- Data Structures. --- Database Management. --- Mathematics. --- Bio-informatics --- Biological informatics --- Information science --- Systems biology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Data base management --- Data services (Database management) --- Database management services --- DBMS (Computer science) --- Generalized data management systems --- Services, Database management --- Systems, Database management --- Systems, Generalized database management --- Electronic data processing --- Computer mathematics --- Discrete mathematics --- Algorism --- Algebra --- Arithmetic --- Information structures (Computer science) --- Structures, Data (Computer science) --- Structures, Information (Computer science) --- File organization (Computer science) --- Abstract data types (Computer science) --- Biosciences --- Sciences, Life --- Science --- Data processing --- Composition --- Mathematics --- Foundations --- Technique --- Computer software. --- Computational complexity. --- Data structures (Computer scienc. --- Complexity, Computational --- Machine theory --- Software, Computer --- Computer systems --- Computer science—Mathematics.
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Molecular biology --- Biologie moléculaire --- Computer simulation --- Congresses. --- Simulation par ordinateur --- Congrès --- Genomics --- Computational Biology --- Sequence Analysis --- Gene Expression --- Genetic Processes --- Genetic Techniques --- Biology --- Genetics --- Biological Science Disciplines --- Investigative Techniques --- Genetic Phenomena --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Computer Science --- Biophysics --- Engineering & Applied Sciences --- Health & Biological Sciences --- Molecular biochemistry --- Molecular biophysics --- Computer science. --- Data structures (Computer science). --- Algorithms. --- Computer science --- Database management. --- Artificial intelligence. --- Bioinformatics. --- Computer Science. --- Algorithm Analysis and Problem Complexity. --- Data Structures. --- Discrete Mathematics in Computer Science. --- Database Management. --- Artificial Intelligence (incl. Robotics). --- Mathematics. --- Bio-informatics --- Biological informatics --- Information science --- Computational biology --- Systems biology --- AI (Artificial intelligence) --- Artificial thinking --- Electronic brains --- Intellectronics --- Intelligence, Artificial --- Intelligent machines --- Machine intelligence --- Thinking, Artificial --- Bionics --- Cognitive science --- Digital computer simulation --- Electronic data processing --- Logic machines --- Machine theory --- Self-organizing systems --- Simulation methods --- Fifth generation computers --- Neural computers --- Data base management --- Data services (Database management) --- Database management services --- DBMS (Computer science) --- Generalized data management systems --- Services, Database management --- Systems, Database management --- Systems, Generalized database management --- Computer mathematics --- Discrete mathematics --- Algorism --- Algebra --- Arithmetic --- Information structures (Computer science) --- Structures, Data (Computer science) --- Structures, Information (Computer science) --- File organization (Computer science) --- Abstract data types (Computer science) --- Informatics --- Science --- Data processing --- Mathematics --- Foundations --- Biochemistry --- Biomolecules --- Computer software. --- Data structures (Computer scienc. --- Computational complexity. --- Artificial Intelligence. --- Complexity, Computational --- Software, Computer --- Computer systems --- Computer science—Mathematics. --- Molecular biology - Computer simulation - Congresses.
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